Comparative study on antioxidative system in normal and vitrified shoots of Populus suaveolens in tissue culture

To explore the physiological and biochemical mechanism of the occurrence of vitrified shoots of Populus suaveolens in tissue culture, the changes in water, chlorphyll, lignin, H₂O₂, phenylalanine ammonialyase (PAL), malonaldehyde (MDA), protective enzymatic systems, and some key enzymes involved in the ascorbate- glutathione cycle were comparatively studied in both normal and vitrified shoots of P. suaveolens. The results show that the lower activities of peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathione reductase (GR) and PAL, and the less contents of chlorphyll, lignin, ascorbate (ASA) and reduced glutathione (GSH) as well as the lower ratios of ASA / DHA and GSH / GSSG are observed in vitrified shoots than in normal ones during the whole culture period. While in comparison with normal shoots, the higher activity of superoxide dismutase (SOD) and the more concentrations of water, H₂O₂, MDA, dehydroascorbate (DHA) and oxidized glutathione (GSSG) are found in vitrified shoots. Statistical analysis indicates that the enhanced activity of SOD and the decreased activities of CAT and POD as well as some enzymes involved in the ascorbate-glutathione cycle might be closely correlated to the accumulation of H₂O₂. The less regeneration of ASA and GSH and the lower capacity of the ascorbate-glutathione cycle observed in vitrified shoots might be due to a significant decrease in APX, MDAR, DHAR and GR activities and a decline in redox status of ASA and GSH. The decreases in chlorphyll content might result in a decline in photosynthesis. The lower activities of POD and PAL could result in the decrease of lignin synthesis and cell wall ligination, which might be the key factor leading to the increase in water content. It is concluded that the deficiency of detoxification capacity caused by the lower capacity of the ascorbate-glutathione pathway and the decreased activity of protective enzymatic system might lead to the large accumulation of H₂O₂ and the enhancement of membrane lipid peroxidation, which might be the main cause leading to the occurrence of vitrifying shoots of P. suaveolens in tissue culture. [Supported by National Natural Science Foundation of China (Grant No. 30271093) and the Foundation of State-designated Base for Biology Researching and Teaching in Beijing Forestry University]     

福建寿宁县兽害猖獗的成因及对策初探

1998～2006年,以寿宁县为调查对象,运用机械抽样法调查样带69条,运用访问调查法调查猎户农户100余户,运用点面互换法调查全县范围内农田作物受害程度。结果表明,全县近半数的村,15%的耕地存在兽害,99%的兽害及其损失源于野猪的活动。分析表明,兽害的成因有种群爆发、物种灭绝和生态系统衰败诸多因素,而人为干扰是各种现象的起因。最后提出治理山区兽害的对策,尤其应加强生态系统恢复和自然保护区建设。     

珠江三角洲陶瓷工业污染区植被受害证据与启示

以长期暴露在大气污染胁迫下的村边林为对象,进行植物群落结构调查、植物受害分析,探讨了陶瓷工业污染排放对植被的影响。调查样地位于广东南海区五星村边坡地的半自然次生林内,乔木层和灌木、草本幼苗层的调查分别在10个10m×10m和4个5m×5m的样方内进行。该森林群落以隆缘桉和尾叶桉数量占优势,其次是乡土树种鸭脚木、泥竹,重要值依次为26.75、17.08、16.27和11.50。隆缘桉和马尾松冠层枝叶完全枯死、脱落,受害程度达100%;其次是泥竹和南岭黄檀受害程度分别为85.1%和68.3%。尾叶桉、朴树、越南山龙眼、阴香、黄荆受害程度中等,在45%—57.5%之间;其它伴生本土种类如鸭脚木、珊瑚树、酒饼叶、等的重要值较低且表现出受害程度较严重。和上层乔木比较,林下植被受污染的伤害大大降低。桉树类树种因其速生、快速郁闭等特点被认为是华南荒山、退化丘陵地区植被恢复的重要种类,但在重度污染地区已面临严重威胁,而某些乡土树种在污染地区则显示出较强的抵御大气污染的能力。本文结果为污染地区森林资源管理,退化生态系统植被恢复之树种选择以及进一步开展乡土树种抵御大气污染胁迫的过程机制与功能特征的研究提供科学基础。     

水泥砼路面改造前的病害处理

本文介绍的是在破损的水泥混凝土路面上进行沥青混凝土路面铺筑时,旧路面的处理方法和施工工艺。     

日本龟蜡蚧在枣树上的发生与危害

１９９６年８月￣１９９７年６月，在陕西大荔县八鱼乡西营村和蒲支县平路庙乡直杜村调查了日本龟蜡蚧在枣树上的分布与危害。结果表明，单叶幼虫数随枣树受害程度的增加而增加，单果重随枣树爱害程度的增加而减少，越冬雌虫的危害造成每股的抽吊数减少，吊长显著变短，每吊的刺树数减少，幼虫和雌成虫在树冠上的分布是阳面（南面）大于阴面（北面），其中树冠中部虫量分布的最多。     

Resistance against oxidative stress in leaves of young beech trees grown in model ecosystems with different soil qualities, elevated CO2, and lachnid infestation

Summary The aim of the present study was to investigate whether the resistance of beech foliage(Fagus sylvatica) against oxidative stress was affected by soil quality, nitrogen or CO₂ fertilisation, or lachnid infestation(Phyllaphis fagi). For this purpose young beech trees were grown for four years in reconstructed calcareous or acidic forest soils in open top chambers under ambient or elevated CO₂ concentrations with two levels of nitrogen fertilisation. At harvest lachnid colonisation was observed, preferentially on leaves from trees in calcareous soil and on leaves from trees fertilised with the high nitrogen level. General leaf characteristics such as pigment concentrations, dry mass, and leaf mass ber area were not affected by the soil type, nitrogen fertilisation or CO₂ regime. Leaves colonised with lachnids displayed slightly increased leaf mass per area. When the stress resistance was challenged by exposure to paraquat — a herbicide inducing oxidative stress — leaves from trees grown on calcareous soil maintained significantly longer membrane integrity and, thus, were better protected against stress than leaves from trees on acidic soil. Other experimental variables had negligible or no effects on the resistance against oxidative stress.      

冰雪灾后粤北山区受损杉木萌条生长规律研究

以位于粤北山区天井山林场的杉木（Cunninghamia lanceolata）人工林中的受损杉木为研究对象,研究了2008年初冰雪灾害后1年内受损杉木的恢复和更新情况。采用标准地调查法,选取4种不同类型受损杉木的标准木（类型Ⅰ：断干高在原树高的1/3内;类型Ⅱ：断干高在原树高的1/3~2/3处;类型Ⅲ：断干高在原树高的2/3以上;类型Ⅳ：倒伏）,监测其自2008年11月—2009年11月期间的萌条高和基径的月生长动态。结果表明：冰雪灾害后的一年内,受损杉木萌条高生长的旺盛期为5—9月份,而基径生长总体呈现初期生长增加明显,后期增速逐渐缓慢的特点。林分密度与受损杉木萌条的生长关系密切,需在灾后杉木人工林重建时给予重点关注。受损Ⅲ型和受损Ⅳ型杉木萌条生长较好,但综合考虑生长和成材效果,建议保留受损Ⅲ型,伐掉受损杉木Ⅰ型、Ⅱ型和Ⅳ型。     

A review of the effect of stresses between lifting and planting on nursery stock quality and performance

This paper presents the experimental evidence on the effects of desiccation, low and high temperatures, rough handling, root loss and storage between lifting and planting on plant quality and performance. The review concentrates on the impact of these stresses on outplanting performance; their effect on seedling properties, such as root growth potential and root electrolyte leakage, is also described. Stresses most often happen in combination but there is relatively little information on their interactions. Examples of desiccation plus rough handling, cold storage plus desiccation, frost damage plus freeze storage and the triple stress of desiccation, heating and rough handling are presented. The limited evidence on the cumulative effect of stresses suggests tentatively that damage from rough handling does not accumulate in the same way as damage from desiccation and prolonged storage. Factors affecting stress resistance are briefly reviewed.There are three impediments to producing a critical review of the literature: experimental treatments, particularly of desiccation and rough handling, can rarely be directly compared; the impact of stress treatments can be modified by a large number of factors, and the levels of stress have seldom been quantified in normal forestry practice. The first two problems also make it difficult to formulate general statements concerning critical thresholds for the stresses. However, instruments are now available for measuring and monitoring stresses between lifting and planting and it is, in theory, possible to model the effects of these stresses to take account of the many factors that modify their impact on seedling survival and growth. This offers the possibility of significant improvements in our ability to predict the effect of stresses between lifting and planting on seedling performance.     

纵坑切梢小蠹对加勒比松的为害

对纵坑切梢小蠹在白云山不同变种加勒比松的为害状况进行了初步调查，掌握了该虫为害松树的特征如：侵入孔部位、侵入孔直径、侵入孔道长度、为害梢直径、为害梢长度等，发现纵坑切梢小蠹对疏叶型本种加勒比松的春梢为害较严重。     

Effects of a severe typhoon on forest dynamics in a warm-temperate evergreen broad-leaved forest in southwestern Japan

The effects of a typhoon on forest dynamics and the response of major tree species were studied in a warm-temperate evergreen broad-leaved forest in southwestern Japan. The strongest typhoon on record (T9313) passed through this region in 1993. Return periods of typhoons over 30 ms⁻¹ in instantaneous wind velocity and T9313 were estimated to be 2.2 and 104.5 years, respectively. Approximately 10% of all stems suffered some damage from T9313 and annual stem mortality rose from 1.3 to 2.7%. The estimated period that the number of stems would fall below 10% of the initial was four years shorter with T9313-class typhoons than without them. Thus, the disturbance by T9313 was not catastrophic at the site although T9313 was an episodic typhoon. The short-term responses of major tree species to T9313 were classified into four types: 1) blunt-response type with little decrease and recruitment of stems in the DBH ≥ 5 cm class (Distylium racemosum), 2) retreat type with larger decrease than recruitment (e.g. Quercus acuta), 3) sharp-response type with a large decrease and much recruitment (e.g. Cinnamomum japonicum), and 4) advance type with less decrease than recruitment (Eurya japonica). Among the four regeneration types classified by previous studies (climax, light-demanding, subcanopy, and few-sapling), the climax and few-sapling types each showed a specific short-term response, the blunt-response and retreat types, respectively, that explains one aspect of the regeneration strategies of each type. On the other hand, the light-demanding or sub-canopy type showed multiple short-term responses, indicating that each regeneration type contains species with various regeneration strategies. In this paper, I analyzed data from the database that was compiled by the Aya Research Team.